Journal: International Journal of Molecular Sciences

Article Title: Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model

doi: 10.3390/ijms232113623

Figure Lengend Snippet: Fluoxetine reduced viral load and inflammatory response in K18-hACE2 mice infected with SARS-CoV-2. ( A ) Experimental design of the study; ( B ) infectious viral loads in the lungs of vehicle- and fluoxetine-treated mice infected with SARS-CoV-2 at day 2 and 6 post-infection expressed as log10 TCID50/mL; ( C ) viral RNA levels in the lungs of vehicle- and fluoxetine-treated mice infected with SARS-CoV-2 at day 2 and 6 post-infection expressed as Lung SARS-CoV-2 RNA load (RNA copies/µL). ( D ) Cytokine and chemokine transcripts in lung tissue at day 2 and 6 post-infection of SARS-CoV-2-infected mice, treated with vehicle or with fluoxetine. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: For in vitro experiments, fluoxetine hydrochloride (Tocris Bio-Techne, Noyal Châtillon sur Seiche, France; CAS Number: 56296-78-7) was dissolved in H 2 O 2 at 10 mM concentration and stored at −20 °C until use.

Techniques: Infection

Journal: International Journal of Molecular Sciences

Article Title: Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model

doi: 10.3390/ijms232113623

Figure Lengend Snippet: Fluoxetine effect on lung levels of ( A ) ceramide 16:0, ( B ) hexosylceramide 16:0, ( C ) hexosylceramide 16:0 / ceramide 16:0 ratio, and ( D ) sphingomyelin 16:0, at day 2 post-infection. *, p < 0.05.

Article Snippet: For in vitro experiments, fluoxetine hydrochloride (Tocris Bio-Techne, Noyal Châtillon sur Seiche, France; CAS Number: 56296-78-7) was dissolved in H 2 O 2 at 10 mM concentration and stored at −20 °C until use.

Techniques: Infection

Journal: International Journal of Molecular Sciences

Article Title: Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model

doi: 10.3390/ijms232113623

Figure Lengend Snippet: Fluoxetine activity on SARS-CoV-2 variants in vitro. ( A ) Activity of fluoxetine (12.5 µM) or mock (water) in Vero E6 cells infected with SARS-CoV-2 (Pasteur, B.1), B.1.1.7 (UK), P1, B.1.617 (Delta) and BA.5 (Omicron) variants measured by assessing cell viability and viral load. ( B ) Antiviral activity of fluoxetine (12.5 µM) measured in A549 cells expressing hACE2-TMPRSS2 infected with SARS-CoV-2 (Pasteur, B.1), B.1.1.7 (UK), P1, B.1.617 (Delta) and BA.5 (Omicron) variants measured by cell viability and viral load. Representative of two to seven independent experiments, data are expressed as mean + SEM. *, p < 0.05, **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: For in vitro experiments, fluoxetine hydrochloride (Tocris Bio-Techne, Noyal Châtillon sur Seiche, France; CAS Number: 56296-78-7) was dissolved in H 2 O 2 at 10 mM concentration and stored at −20 °C until use.

Techniques: Activity Assay, In Vitro, Infection, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model

doi: 10.3390/ijms232113623

Figure Lengend Snippet: Fluoxetine inhibits SARS-CoV-2 infection of 2D human airway cells. ( A ) Representative images of immunofluorescent stainings of mNeon Green SARS-CoV-2 infected airway cultures. Acetylated tubulin (AcTUB, red) stains cell ciliates, phalloidin-coupled Alexa Fluor 647 stains F-actin (purple) and DAPI stains nuclei (blue). Scale bar = 10 µm. ( B ) Live virus titre measured on apical washes 72 h after infection with mNeon Green SARS-Cov-2. Data from three independent experiments on two independent donors, expressed as mean ± SEM, **** p < 0.0001.

Article Snippet: For in vitro experiments, fluoxetine hydrochloride (Tocris Bio-Techne, Noyal Châtillon sur Seiche, France; CAS Number: 56296-78-7) was dissolved in H 2 O 2 at 10 mM concentration and stored at −20 °C until use.

Techniques: Infection, Virus

Journal: Neuron

Article Title: The Serotonergic Raphe Promote Sleep in Zebrafish and Mice

doi: 10.1016/j.neuron.2019.05.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For optogenetic experiments in conjunction with fluoxetine injection, 10-20 mg/kg fluoxetine (BML-NS140-0050, Enzo Life Sciences, Inc., NY, USA) was delivered by i.p. injection 30-60 min before the start of the experiment (i.e. 30-60 min before lights on or off).

Techniques: Recombinant, Plasmid Preparation, Mutagenesis, Software